项目名称： 大麦5HS染色体上叶锈病成株抗性基因（LR-APR）的精细定位与克隆

项目编号： No.31201212

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 徐延浩

作者单位： 长江大学

项目金额： 25万元

中文摘要： 叶锈病是大麦的重要病害，利用成株抗性培育具持久抗性的大麦品种是控制叶锈病的根本措施。LR-APR是大麦5HS染色体上成株抗性QTL位点的主效基因，在WABAR2482×mpadour F2群体中被解析为显性单基因，定位在2.0 cM区间内。在此基础上，本研究拟用大麦基因组测序信息，加密LR-APR基因区间分子标记，在上述群体中对该基因进行精细定位，筛选该基因共分离标记，比对5H染色体序列，构建该基因物理图谱，预测候选基因，比较候选基因亲本单体型及表达差异，缩小候选基因范围。构建抗性亲本的TAC文库，利用候选基因特异分子标记筛选TAC文库，对阳性克隆测序并转化感病品种。比较分析LR-APR基因位点在抗性品种中的多态性，明确5HS上是否存在成株抗性的多基因家族或LR-APR复等位基因。LR-APR基因的精细定位和克隆，将促进对大麦叶锈病成株抗性机制的理解，为大麦抗叶锈病育种提供信息。

中文关键词： 大麦叶锈病；5HS染色体；LR-APR基因簇；精细定位；候选基因

英文摘要： Leaf rust caused by the fungus Puccinia hordei is one of the most significant diseases of barley worldwide. Adult plant resistance (APR) is considered potentially more durable for controlling barley leaf rust than seedling Rph (Resistance to Puccinia hordei) genes. The most preferable and economical method for controlling barley leaf rust is to develop and utilize the durable host resistance. LR-APR is the major gene of QTL on barley chromosome 5HS controlling adult plant resistance to Puccinia hordei, which was dissected into a dominant gene in F2 population of WABAR2482 ×ompadour and was mapped in a 2.0 cM interval (with 0.7 cM from marker bPb-0837 and 1.3 cM from marker EBmag0833). In this study, the barley genome sequence will be used to develop new markers in the LR-APR gene interval. The F2 population with 2600 individuals from WABAR2482 ×ompadour will be used to construct a fine mapping of LR-APR gene and to identify markers cosegregated with LR-APR. The cosegregated markers will be used to screen the sequences from barley 5HS chromosome to construct the physical map of LR-APR gene. The candidate genes will be predicted by bioinformatics approach. The haplotype and the expression patterns of the candidate genes will be analyzed in the DH population of Pompadour ×tirling to narrow the scope of candid

英文关键词： Barley leaf rust；chromosome 5HS；LR-APR gene cluster；fine mapping；candidate gene