项目名称： 应用宏基因组学探讨抑郁症肝郁乘脾的发生机制

项目编号： No.81503475

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 于林

作者单位： 广州医科大学

项目金额： 18万元

中文摘要： “木乘土”是中医五行学说的重要理论，但其科学内涵尚未阐明。诸多证据表明：肠道微生物在抑郁症发病中起到重要作用。中医学认为肝郁乘脾（木乘土）是抑郁症的重要病机和常见证候。我们前期研究发现抑郁症肝郁乘脾患者肠道菌群结构发生显著的变化，而给予逍遥丸治疗后可促进其肠道菌群异常的恢复。因此推测肠道菌群的稳态失衡可能促进了肝郁乘脾的发生发展过程。本项目拟采用病证结合方法，以逍遥丸作为干预手段，应用宏基因组学方法，首先在临床上探讨抑郁症肝郁乘脾患者肠道菌群的变化特征，其次利用抑郁症转基因小鼠制作动物模型，探讨小鼠模型肠道菌群的变化特征；再次，应用粪菌移植技术，利用无菌小鼠探讨肠道菌群对肝郁乘脾发生的影响。最后，利用生物信息学方法进行整合分析，阐明肠道菌群的稳态失衡在抑郁症肝郁乘脾发生中的作用，从肠道微生物角度揭示中医“木乘土”理论的科学内涵，并明确逍遥丸对肠道微生物的调节机制，为其临床应用提供科学依据。

中文关键词： 抑郁症；肝郁乘脾；宏基因组学；肠道菌群；逍遥丸

英文摘要： The wood over-restraining the earth, the important theory about the five elements theory of traditional Chinese medicine (TCM), has yet to require further elucidation for its scientific connotation. Previous studies have presented evidence that gut microbiota has play an important role in the occurrence of depression. Liver depression over-restraining the spleen, which is the same as the wood over-restraining the earth, is the important pathogenesis and common syndrome in TCM. A previous investigation by us had found that remarkable changes took place in the construction of gut microbiota among the depression patients with liver depression over-restraining the spleen, and it could be recover by the treatment of Xiaoyao Pill. Accordingly, we theorize that the abnormal gut microbiota may play an important role in the occurrence of liver depression over-restraining the spleen. In this project, we aim to investigate the change characteristics of gut microbiota with liver depression over-restraining the spleen in depression patients clinically, and in the making animal model of the transgenic mouse with depression, by adopting the method of disease integrating with syndrome, the intervention means of Xiaoyao Pill, and the technology of metagenomic; eventually, conducting bioinformatics method for integrated analysis, we will ascertain how the unbalance of homeostasis in gut microbiota works in the development of depression with liver depression over-restraining the spleen, reveal the scientific connotation of the wood over-restraining the earth, and confirm the regulation mechanism of gut microbiota in the treatment of depression by Xiaoyao Pill, intending to provide scientific evidence for clinical adoption.

英文关键词： depressive disorder ;liver stangation and spleen defieiency;metagenomics;intestinal flora;Xiao-yao pill