项目名称： 新型的软骨基质蛋白/螺旋纳米管仿生纳米基质对于骺板软骨再生的研究

项目编号： No.81501892

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 陈宇鹏

作者单位： 西安交通大学

项目金额： 18万元

中文摘要： 在医学临床上，未成年人骨骺骨折之后的骺板损伤可导致长骨生长畸形，造成身体功能障碍。在生物学研究中，如何修复骺板软骨是一个亟待解决的科学问题。本课题组目的是研究抑制骺板软骨骨化、促进软骨再生的生物学机理，并评估软骨基质蛋白3（MATN3）/螺旋纳米管（RNT）仿生纳米基质抑制成骨和促进软骨分化方面的效果。为实现此目的，本课题组依据前期研究成果和关键步骤的预实验，采用注射方法将体内自组装的MATN3/RNT仿生纳米基质应用于大鼠骨骺骨折模型，进而评估该制剂在骺板软骨再生的效果；并发展了MATN3的多重、靶向生长因子递送的技术，明确了其促进软骨分化的功能。因此，本课题有望为骺板损伤提供一种新型、高效、无创（或微创）的治疗思路，具有重要的研究价值和临床转化潜力。

中文关键词： 软骨损伤；软骨修复；纳米材料；生长因子

英文摘要： Growth plate fracture in children represents a significant problem in clinics, since bony bridge formation in growth plate cartilage can result in angular deformities or longitudinal shortening in the long bone. The objective of this proposal is to evaluate therapeutic effects of nano-matrix self-assembled from matrilin-3 (MATN3) and rosette nanotube (RNT) in a preclinical growth plate fracture model, as well as identify the underlying mechanism. Based on our published research and preliminary results, we hypothesized that MATN3 and RNT can be injected into the fracture site, and they can self-assemble into nano-matrix at the target area; and selectively promote chondro-lineage cell growth and enhance chondrogenesis, while inhibit other cell growth (such as osteoblasts and vascular endothelial cells), thereby, prevent bony bridge formation and improve growth plate cartilage regeneration; additionally, MATN3 can be utilized to bind and deliver growth factors for improving chondrogenesis and cartilage regeneration. The rationale that underlies the proposal is that once the injectable approach is developed to achieve synergistic effects of bony bridge formation and promoting chondrogenesis, it can be used as a minimally invasive and highly effective procedure to treat growth plate fracture.

英文关键词： Cartilage Damage;Cartilage Repair;Nanomaterials;Growth Factors