项目名称： 果汁中脂环酸芽孢杆菌磁性荧光量子点快速检测理论方法

项目编号： No.31501499

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 王周利

作者单位： 西北农林科技大学

项目金额： 20万元

中文摘要： 脂环酸芽孢杆菌是果汁产业中关键的质量安全因子，研究建立高效、快速的检测方法，及时识别、发现与快速检测脂环酸芽孢杆菌的污染，一直是中国果汁国际化发展的技术瓶颈之一。本项目通过磁性纳米微球偶联荧光量子点，制备获得性能优良的磁性荧光量子点复合材料，对其分子组成及化学结构进行解析，揭示磁性纳米微球和荧光量子点耦合的作用方式与结合位点；优化建立基于磁性荧光量子点复合材料的脂环酸芽孢杆菌多克隆抗体偶联固定化方法，制备获得兼具磁性分离及荧光检测特性的功能化磁性荧光量子点复合材料，分析评价其结构组成和生物性能，明确多克隆抗体固定化的作用位点与分子机制；确定功能化磁性荧光量子点复合材料对脂环酸芽孢杆菌磁性分离的特异性、最佳作用方式与范围，通过线性方程和最小二乘法优化建立脂环酸芽孢杆菌快速检测和定量分析模型，明确快速检测方法的灵敏度、准确性，系统构建果汁中脂环酸芽孢杆菌磁性荧光量子点快速检测技术方法体系。

中文关键词： 果汁；脂环酸芽孢杆菌；磁性荧光量子点；快速检测；理论方法

英文摘要： Alicyclobacillus spp. are one of the most important quality and safety factors of fruit juice and have become a major issue in the fruit juice processing industry. The development of rapid and accurate methods for the detection and identifying of Alicyclobacillus spp. in fruit juice products has become a focus of investigation. In this project, magnetic-fluorescent quantum dots will be prepared based on the magnetic nanoparticles and fluorescence quantum dots, the coupling way and binding sites are assisted by analysis of the molecular composition and chemical structure. The immobilization methods and conditions of specific anti-Alicyclobacillus antibodied onto magnetic nanoparticles will be optimized, the functional magnetic composite materials, which can be used for the immunomagnetic separation and detection of Alicyclobacillus spp., will be generated. Meanwhile, the molecular mechanism for the specific anti-Alicyclobacillus antibodies binding to magnetic-fluorescent quantum dots are revealed by the analysis of structure compositions and biological properties. The most suitable function range, methods and specificity of functional magnetic composite materials for the magnetic separation of Alicyclobacillus spp. are evaluated, and the detection limit and accuracy are comfirmed. Based on the linear equation and least square method, the model will be establised for rapid detection and quantitative analysis of Alicyclobacillus spp. in fruit juice. Finally, the theoretical and methodology systems for magnetic separation and rapid detection of Alicyclobacillus spp. from fruit juice by magnetic-fluorescent quantum dots will be established. This work is believed to be of both academic interest and practical importance for monitoring Alicyclobacillus spp. in fruit juice and other beverage industry.

英文关键词： fruit juice;Alicyclobacillus spp.;magnetic-fluorescent quantum dots;magnetic separation;the method and mechanism