项目名称： 基于水母发光蛋白的植物低温胁迫Ca2+信号遗传分析

项目编号： No.U1204302

项目类型： 联合基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 张国增

作者单位： 河南大学

项目金额： 31万元

中文摘要： 低温胁迫严重影响着作物的产量和品质。低温刺激后Ca2+浓度迅速升高，抑制Ca2+浓度升高则破坏冷驯化，因此研究Ca2+介导的低温信号转导机制具有重要意义。但是目前尚不清楚什么基因参与了低温Ca2+信号转导，也未见低温信号与Ca2+信号交互作用（crosstalk）的报道。本项目以基因组学研究为基础，利用生物信息学分析拟南芥中低温调控的基因，选取低温诱导或抑制的基因并获得T-DNA插入拟南芥。将活体Ca2+探针——水母发光蛋白转入突变体中，检测低温刺激后突变体细胞Ca2+浓度变化。对Ca2+浓度特异变化突变体利用生理学、分子生物学和遗传学方法解析突变基因功能。然后选择表型变化相似的突变体构建双突变体或三突变体，多途径多方法解析基因的空间关系，建立低温Ca2+信号转导的网络框架。本研究将初步建立Ca2+信号在低温信号转导中的时空作用模式，为理解低温信号转导机制奠定坚实的细胞学和遗传学基础。

中文关键词： 钙离子信号；低温胁迫；水母发光蛋白；拟南芥；

英文摘要： Low temperature is an important environmental factor influencing crop quality and productivity. The immediate response to low temperature in plant is a transient increase in intracellular Ca2+ concentration, and the inhibition of Ca2+ response would hinder cold acclimation. Therefore, it is important to study the mechanism of Ca2+ mediated low-temperature signal transduction. However, it is not yet clear what genes are involved in low-temperature Ca2+ signal transduction, and the crosstalk between low temperature and Ca2+ signaling has rarely been reported. By analysis of low temperature regulated genes from Arabidopsis microarray data, we would select low temperature induced or inhibited genes and obtain the corresponding T-DNA insertion mutants. To address the relationship between those genes and Ca2+, the aequorin would be transformed into the mutants, and then cold-triggered Ca2+ responses would be measured. The functions of the mutant gene which shows the specific changes of Ca2+ would be analyzed through physiology, molecular biology and genetics methods. The relationships of the genes would be analyzed by constructing double or triple mutants which were obtained by crossing similar phenotype mutants. This project will be helpful to define the intermediates of Ca2+ -mediated signal transduction into nucleu

英文关键词： Calcium signalling；Cold stress；Aequorin；Arabidopsis thaliana；