Electrochemical communication is a mechanism that enables intercellular interaction among bacteria within communities. Bacteria achieves synchronization and coordinates collective actions at the population level through the utilization of electrochemical signals. In this work, we investigate the response of bacterial biofilms to artificial potassium concentration stimulation. We introduce signal inputs at a specific location within the biofilm and observe their transmission to other regions, facilitated by intermediary cells that amplify and relay the signal. We analyze the output signals when biofilm regions are subjected to different input signal types and explore their impact on biofilm growth. Furthermore, we investigate how the temporal gap between input pulses influences output signal characteristics, demonstrating that an appropriate gap yields distinct and well-defined output signals. Our research sheds light on the potential of bacterial biofilms as communication nodes in electrochemical communication networks.

翻译：电化学通讯是一种使细菌群落内细胞间发生相互作用的机制。细菌通过利用电化学信号实现种群层面的同步并协调集体行为。本研究探讨了细菌生物膜对外源钾离子浓度刺激的响应。我们在生物膜中特定位置引入信号输入，并观察其通过中间细胞（即放大与中继信号的媒介）向其他区域的传导过程。当生物膜区域受到不同类型输入信号作用时，我们分析了输出信号特征，并探究这些信号对生物膜生长的影响。此外，我们进一步研究了输入脉冲之间的时间间隔如何影响输出信号特性，结果表明适当的时间间隔能够产生清晰明确的输出信号。本研究揭示了细菌生物膜作为电化学通讯网络中通讯节点的潜在可能性。