Background, enhancing interoperability of bioinformatics knowledge bases is a high priority requirement to maximize data reusability, and thus increase their utility such as the return on investment for biomedical research. A knowledge base may provide useful information for life scientists and other knowledge bases, but it only acquires exchange value once the knowledge base is (re)used, and without interoperability the utility lies dormant. Results, in this article, we discuss several approaches to boost interoperability depending on the interoperable parts. The findings are driven by several real-world scenario examples that were mostly implemented by Bgee, a well-established gene expression database. Moreover, we discuss ten general main lessons learnt. These lessons can be applied in the context of any bioinformatics knowledge base to foster data reusability. Conclusions, this work provides pragmatic methods and transferable skills to promote reusability of bioinformatics knowledge bases by focusing on interoperability.

翻译：背景：增强生物信息学知识库的互操作性是最大化数据可重用性的高优先级需求，从而提升其效用，例如提高生物医学研究的投资回报率。一个知识库可能为生命科学家及其他知识库提供有用信息，但只有在知识库被（重）使用时才获得交换价值，而缺乏互操作性会使效用处于休眠状态。结果：在本文中，我们讨论了基于可互操作组件的若干提升互操作性的方法。这些发现由多个真实场景案例驱动，其中大部分由Bgee（一个成熟的基因表达数据库）实现。此外，我们总结了十条主要的一般性经验教训。这些经验教训可应用于任何生物信息学知识库的背景中，以促进数据的可重用性。结论：本工作通过聚焦互操作性，提供了促进生物信息学知识库可重用性的实用方法和可迁移技能。