Background, enhancing interoperability of bioinformatics knowledge bases is a high priority requirement to maximize data reusability, and thus increase their utility such as the return on investment for biomedical research. A knowledge base may provide useful information for life scientists and other knowledge bases, but it only acquires exchange value once the knowledge base is (re)used, and without interoperability the utility lies dormant. Results, in this article, we discuss several approaches to boost interoperability depending on the interoperable parts. The findings are driven by several real-world scenario examples that were mostly implemented by Bgee, a well-established gene expression database. Moreover, we discuss ten general main lessons learnt. These lessons can be applied in the context of any bioinformatics knowledge base to foster data reusability. Conclusions, this work provides pragmatic methods and transferable skills to promote reusability of bioinformatics knowledge bases by focusing on interoperability.

翻译：背景，增强生物信息学知识库的互操作性是提高数据可重复使用性的紧迫需求，因此可以增加生物医学研究的投资回报率。知识库可以为生命科学家和其他知识库提供有用的信息，但只有在知识库被（重新）使用并具有互操作性时，它才会获得交换价值，而没有互操作性，其实用性便一直潜在。结果，本文讨论了多种不同的方法来提高互操作性，取决于可互操作的部分。这些发现是由Bgee实施的几个真实场景示例驱动的，Bgee是一个非常成熟的基因表达数据库。此外，我们还讨论了十个主要的普适性经验教训。这些经验教训可在任何生物信息学知识库的背景下应用，以促进数据可重复使用性。结论，本文提供了实用的方法和可转移的技能，以关注互操作性来促进生物信息学知识库的可重复使用性。