项目名称： 两栖动物适应高海拔生境的分子基础——基于抗氧化多肽组学的新探索

项目编号： No.31460571

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 动物学

项目作者： 王滢

作者单位： 云南民族大学

项目金额： 50万元

中文摘要： 目前，生物适应高海拔的分子机制一直是国际上的一个研究热点，但鲜有研究报道尝试从蛋白质/多肽组学差异层面进行探索。在前期的工作中，我们选取了一对臭蛙姐妹物种：云南臭蛙和务川臭蛙，二者的生境有一个非常明显的差异：前者生活在高海拔生境中，紫外照射强烈，而后者却是洞穴物种，无光照。因此，我们推测在环境压力的驱使下，二者皮肤中抗氧化多肽在多方面必然存在较大差异。我们通过分离纯化技术，初步发现云南臭蛙皮肤抗氧化多肽丰度要远远大于务川臭蛙。本项目旨在通过多肽组学结合基因组学、功能蛋白质组学手段，比较云南臭蛙和务川臭蛙皮肤中的抗氧化多肽在丰度、结构、翻译后修饰类型及抗氧化活性方面的不同，同时，研究其结构-功能关系，阐明其抗氧化的分子基础，最终为理解物种适应高海拔生境的分子基础提供多肽组学层面的线索，同时为我国新型抗氧化剂的开发提供具有自主知识产权的先导分子。

中文关键词： 高海拔；分子基础；两栖动物；抗氧化肽组学

英文摘要： The genetic basis of adaptation to high-elevation life have been well characterized for many species, however, little is known for amphibians, besides, the information provided by proteomic or peptidomic basis is little known. In order to investigate the molecular basis of amphibians' skins to adaptation the UV-exposure surroundings, we chose a pair of amphibians: Odorrana andersonii and O. wuchuanensis, the former frog lived in high-altitude surroundings with extensive UV exposure; but, in contrast, the latter frog live in cave and without any lights. Based on this observation, we assumed their skin antioxidant peptide (AOP) systems should differ in many aspects. Through the combinational methods of peptidomics, genomics and functional annotations, we found the the AOPs' diversity of O. andersonii is much higher than that of O. wuchuanensis. This program is aiming to compare the difference of skin antioxidant peptides between the high-altitude and caved amphibians in the following aspects: diversity, structure, types of posttranslational modifications and antioxidant activities. Furthermore, in order to elucidate the molecular basis of their antioxidant activities, the research on the relationship of structure-function will be performed. In all, this program will provide novel clues of peptidomics to understand molecular basis of amphibians' adaptation to high-elevation life, besides, novel templates with property rights for the explore of new antioxidants will be provided.

英文关键词： high-altitude;molecular basis;amphibians;antioxidant peptidomics