项目名称： 基于微加工生物芯片的细胞共培养系统研究线粒体转移在干细胞修复心肌细胞中的作用

项目编号： No.81500227

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 刘秋英

作者单位： 暨南大学

项目金额： 18万元

中文摘要： 干细胞移植治疗心血管疾病取得了一定的疗效，但由于对于干细胞的作用机制的不了解，阻碍了干细胞移植治疗的临床应用。为了研究线粒体转移在干细胞移植治疗中的作用及机制，本项目以体外培养的干细胞和心肌细胞微研究对象，采用微加工技术构建双通道微流体系的生物芯片，促进干细胞和心肌细胞在培养过程中形成纳米微管通道结构，观察分析线粒体在纳米微管通道转移的过程；建立H2O2氧化胁迫和LPS诱导凋亡的心肌细胞受损模型，研究间充质干细胞共培养后发生线粒体转移后对心肌细胞的修复作用，明确线粒体转移的修复作用；并进一步采用细胞松弛素D处理和沉默驱动蛋白KHC，研究微管和微丝结构对线粒体转移的影响，阐明线粒体转移的机制。为明确干细胞移植作用机制奠定基础，推进干细胞治疗心血管疾病的临床应用。

中文关键词： 间充质干细胞；心肌细胞；细胞培养；线粒体转移；纳米微管通道

英文摘要： Stem cell transplantation therapy of cardiovascular disease has certain effect, but the clinical application was hindered because the understanding of the mechanism of stem cells transplantation is limited. In order to study the role of mitochondria transfer in stem cell transplantation therapy, this research is focused on co-culture in vitro of cardiomyocytes and mesenchymal stem cells, using a microfluidics-based two-channel biochip by fabrication, which improving formatting tunneling nanotube. The process of tunneling nanotube forming and mitochondrial transfer will be observed and analyzed. The repair function of mitochondria transfer will be evaluated by cell membrane potential and apoptosis through establishing H2O2 oxidation stress and LPS induce the apoptosis of myocardial cell damage model. Further, by deal with cytochalasin D and silence of KHC gene, the influence of microtubule and microfilament structure on mitochondria transfer was declared. This research will provide significant reference data for the mechanisms of stem cell transplantation, and promote the clinical application of stem cell therapy for cardiovascular disease.

英文关键词： mesenchymal stem cell;cardiomyocyte;cell culture;mitochondria transfer;tunneling nanotube