项目名称： 番木瓜籽中硫代葡萄糖苷降解规律及其产物异硫氰酸苄酯BITC抑菌机理的研究

项目编号： No.31501532

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 朱华平

作者单位： 天津科技大学

项目金额： 18万元

中文摘要： 硫代葡萄糖苷是一种广泛存在于植物中的次级代谢产物，由于硫代葡萄糖苷及其异硫氰酸苄酯有多种生理活性，如抗氧化及抑菌等，因而近年来引起国内外学者的广泛关注。本课题以番木瓜加工过程中的废弃物—番木瓜籽为研究对象，剖析番木瓜籽中硫代葡萄糖苷的降解规律，并建立降解动力学模型。在此基础之上，对其降解产物异硫氰酸苄酯的抑菌活性及量效关系进行深入挖掘，诠释其对致病菌菌体细胞结构与功能、物质代谢系统及能量代谢系统的影响规律，阐明异硫氰酸苄酯抑制致病菌生长的作用机理。在明晰异硫氰酸苄酯活性结构基础上，重点剖析异硫氰酸苄酯结构与其抑菌活性之间的构效关系。最终明确硫代葡萄糖苷的降解规律与异硫氰酸苄酯抑菌机理及构效关系，为番木瓜籽高值化利用奠定一定的理论基础，并为开发异硫氰酸苄酯新型天然食品防腐剂提供科学依据。

中文关键词： 天然食品添加剂；构效关系；作用机制；植物提取物

英文摘要： Glucosinolates (GS) are secondary metabolites which were widely existed in plants. GS and its degradation products of benzylisothiocyanate (BITC) are attracted extensive attention in recently years due to their physiological activity, such as antioxidant and anti-bacteria activity. The processing waste of papaya seeds were used as research material in current research. Then the degradation rules of GS were analysed and the degradation kinetic model equation was established. On the basis of study mentioned above, the bacteriostatic activity of BITC and dose-response relationship were been clarified. Moerover, the impact of BITC on structure and function, metabolism system and energy system of pathogenic bacterial could be interpreted. Furthermore, the relationship between structure of BITC and bacteriostatic activity was been in-depth study. Finally the relationship among the degradation pattern of glucosinolate, antibacterial mechanism and structure-function of isothiocyanates could be explained. The result could provide the theoretical foundation for high value using of papaya seeds and the scientific basis for the exploitation of BITC as natural food preservative.

英文关键词： natural food additive;structure-activity relationship ;mechanism;plant extracts