Deep-water biological observation is essential for understanding marine organisms and their interactions with the environment. However, conventional optical and acoustic approaches can introduce stimuli that alter animal behavior and bias biological observations. This paper proposes a fluorescence-enhanced whisker array sensing system that pinpoints underwater hydrodynamic sources through local optical readout rather than direct source imaging. Five spatially oriented whiskers, fabricated with nitinol cores and fluorescent urethane shells, are integrated with ultraviolet excitation and a monocular camera. Image enhancement and segmentation are applied to track the whisker deformation. A lightweight convolutional neural network captures temporal and cross-whisker features from 2 s sequences to estimate source localization. Pool experiments achieve a mean spatial localization error of 88 mm, with 73.5 mm in radius and $2.5^\circ$ in angle, across a test region of 600 mm with $\pm30^\circ$. Real-time localization of a moving thruster demonstrates the capability of the proposed method in dynamic scenarios, highlighting its potential for integration into underwater robots for hydrodynamic source detection, localization, and tracking in low-light environments.

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