项目名称： 巨紫荆耐高盐相关微小RNA及其靶标的研究

项目编号： No.31501045

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 遗传学与生物信息学、细胞生物学

项目作者： 朱柳村

作者单位： 上海大学

项目金额： 20万元

中文摘要： 巨紫荆是当前广泛用于行道树及绿化点缀的树种，且本身有微弱的盐抗性，如能加强其耐盐的能力，将使其成为优秀的盐碱地区绿化树种。针对该树种应用前景广泛且耐盐相关基因表达调控机制不明确的状况，根据微小RNA及与靶标基因的调控作用关系的理论，本项目在前期已对巨紫荆转录组、微小RNA组和降解组进行测序，建立了巨紫荆功能基因、微小RNA及与受微小RNA调控的靶标基因的遗传信息数据库，并在构建了功能基因和微小RNA的相互作用网络的基础上，采用将转录组、微小RNA组和降解组高通量测序分析法三者相结合的网络构建方法，对高盐胁迫下和正常条件下生长的巨紫荆幼苗根和叶的微小RNA及其靶标的表达情况进行研究，最终获得巨紫荆根和叶中表达的耐高盐相关的微小RNA数据库、功能基因数据库及调控网络，并通过分子生物学实验筛选出机制明确的靶标，对阐明耐高盐调控作用机制和培育适应高盐条件下生长的巨紫荆新品种提供参考。

中文关键词： 植物；高通量测序；非编码RNA；表达分析；调控网络

英文摘要： Cercis gigantea is an important garden plant widely grown in China with weak salt resistance. Furthermore, it will be more suitable for growing in salty region with the improvement of salt tolerance. However, little genetic information of C. gigantea is available at present. Since C. gigantea had widely used prospect, we have first finished the transcript, miRNA and degradome sequencing of C. gigantea and established the genetic databases and interaction network of genes, miRNAs and the targets of the miRNAs according to the corresponding relation of miRNA and their targets. The aim of this study is focused on the genetic regulatory network of miRNAs and their targets response to salt stress. We plan to investigate differential expression level of miRNAs and their targets response to salt stress in the different tissues of C. gigantea by comparing the seedlings grown in the salt stress and normal condition using the combination of the methods of transcript, miRNA and degradome sequencing and analysis. Finally, we build the database and regulatory network of miRNAs and their targets of molecular experimental validation under salt stress. Our study provides important platform for not only breeding new type of C.gigantea resistant high salinity but also the regulatory mechanism of salinity tolerance.

英文关键词： Plant;High-throughput sequecing;non-coding RNA;Expression analysis;Regulatory network