项目名称： 基于肝细胞色素P450酶表达调控的竹叶柴胡“疏肝解郁”物质基础及机理研究

项目编号： No.81503200

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 闫婕

作者单位： 成都中医药大学

项目金额： 18万元

中文摘要： 本课题在前期研究结果“竹叶柴胡地上、地下部分均可保肝，但物质基础不同”的基础上，从“疏肝解郁”的中医内涵出发，结合现代药理学理论，认为P450酶的活性与表达和肝的代谢功能密切相关，从而提出“P450的表达调控-疏肝解郁功效”假说。根据直接给药组与造模组对大鼠P450酶活性影响的一致性，验证该假说的科学性，采用cocktail、real-time PCR和western blotting法测定P450酶活性、基因和蛋白的表达量，建立一种评价中药“疏肝解郁”功效的新方法，以此法对竹叶柴胡及其所含不同大类成分进行功效评价，探索竹叶柴胡活性物质基础及作用机理。上述研究成果，将为西南地区习用竹叶柴胡安全、有效、稳定、可控的合理药用提供依据，为科学评价柴胡属其他植物的药用价值及资源开发利用奠定基础。

中文关键词： 疏肝解郁；P450；竹叶柴胡；物质基础；作用机制

英文摘要： This paper considers the activity and expression of P450 enzyme is closely related to the hepatic metabolism, and then proposed the hypothesis that the expression and regulation of P450 can evaluate the efficacy of “relieving-qi-stagnancy” from the connotation of TCM theory with modern pharmacology theory. First, to verify the hypothesis of the scientific nature, according to the consistency of the results of P450 activity between the drug group and the model group. Second, to establish a new method to evaluate the relieving-qi-stagnancy efficacy of Chinese medicine by determining the activity of enzyme and the expression of gene and protein by Cocktail, Real-time PCR and Western blotting. Then, we can use the above method to explore the effectiveness, the material basis and mechanism on B. marginatum in order to provide a reasonable basis of clinical use for ensure safe, effective, stable and controllable. It also can lay the foundation not only for the scientific evaluation of the medicinal value of other Bupleurum. plants but also for the resource development and utilization.

英文关键词： relieving-qi-stagnancy;P450;Bupleurum marginatum;material basis;mechanism