In this research-to-practice paper we present a survey that can be used to assess students' AI knowledge. As the use of artificial intelligence (AI), including generative artificial intelligence (GenAI), has proliferated, so has the need to educate students about the topic. A range of AI literacy frameworks have been proposed, outlining the essential knowledge that students should have. Alongside, different ways of assessing AI knowledge have been developed. As yet, there is a lack of assessment instruments capable of evaluating multiple forms of student knowledge, including technical concepts, practical applications, and ethical concerns about AI use. In this article, we present a study implementing a comprehensive instrument to assess AI knowledge. The instrument combines measures from multiple scales to capture a range of literacy features and actual knowledge. We implemented the instrument in a higher education setting to assess its viability and usefulness and found that the instrument exhibited useful diagnostic capabilities and was able to identify common misconceptions among students. Although students performed well overall, there was a significant misunderstanding of how AI, especially GenAI systems, work. It also identified a lack of higher-level knowledge. The instrument is publicly available for use by others. We foresee its usefulness as a diagnostic that goes beyond understanding students' attitudes and perceptions of AI and GenAI use and tests multiple aspects of students' knowledge and conceptual understanding. This can enable the development of targeted instruction.

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