This study investigates whether predicted bounding boxes are reliable for determining where small-object segmentation should be performed without risking false negatives outside those boxes. The investigation leverages adenovirus particles as small objects, YOLO26x for detection, YO-LO26x-Sem for segmentation, and Mask R-CNN for both detection and segmentation. Segmenta-tion-model predictions were matched to ground-truth adenovirus masks or reference objects using their centroids, with ground-truth mask centroids preferred over ground-truth bounding-box centroids as references for matching. Matched predicted-mask centroids were considered as inside at least one detector-predicted bounding box or outside all such boxes, while ground-truth masks without matched predictions were recorded as having no matched segmentation. For the YOLO26 models, 34 reference objects (47.89%) had matched segmentation centroids inside at least one pre-dicted box, 8 (11.27%) had centroids outside all predicted boxes, and 29 (40.85%) had no matched predicted segmentation. For the Mask R-CNN model, the corresponding counts were 69 (97.18%), 0 (0.00%), and 2 (2.82%). These preliminary experiments demonstrated that restricting segmentation to detector-predicted bounding boxes risks excluding matched segmentation predictions outside those boxes, potentially introducing false negatives.

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