Industrial deployments increasingly rely on Open Platform Communications Unified Architecture (OPC UA) as a secure and platform-independent communication protocol, while private Fifth Generation (5G) networks provide low-latency and high-reliability connectivity for modern automation systems. However, their combination introduces new attack surfaces and traffic characteristics that remain insufficiently understood, particularly with respect to machine learning-based intrusion detection systems (ML-based IDS). This paper presents an experimental study on detecting cyberattacks against OPC UA applications operating over an operational private 5G network. Multiple attack scenarios are executed, and OPC UA traffic is captured and enriched with statistical flow-, packet-, and protocol-aware features. Several supervised ML models are trained and evaluated to distinguish benign and malicious traffic. The results demonstrate that the proposed ML-based IDS achieves high detection performance for a representative set of OPC UA-specific attack scenarios over an operational private 5G network.

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