In this paper, we incorporate the Dirichlet--multinomial distribution into the generalized linear latent variable model (GLLVM) framework to accommodate high-dimensional, overdispersed compositional count data and capture complex covariation among taxa with a low-rank structure. Advances in high-throughput sequencing technologies have generated large amounts of microbiome count data, stimulating research into their associations with human diseases and traits. Such data are compositional and often highly sparse for various reasons, posing challenges for statistical analysis. Conventional zero-replacement approaches ignore the underlying causes of zeros, further complicating the analysis. We therefore propose a zero-inflated Dirichlet--multinomial latent variable model (ZIDM-LVM). Since the marginal likelihood has no closed-form expression, we develop a variational approximation (VA) approach for efficient parameter estimation. Simulation studies and applications to two microbiome datasets are presented to evaluate the performance of the proposed method.

翻译：暂无翻译