项目名称： 基于力学效应的微悬臂梁传感技术在细胞活性监测上的研究

项目编号： No.11502265

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 吴尚犬

作者单位： 中国科学技术大学

项目金额： 22万元

中文摘要： 寻找选择性高、毒副作用小的抗癌药一直是抗肿瘤新药研究的重点之一。本世纪初发展了一种基于力学效应的微悬臂梁生化传感技术，传统的微梁传感都是基于表面应力的变化或者共振频率的漂移来实现检测。除此之外，我们还发现活细胞的存在导致了微梁明显的涨落，当细胞的活性在外界条件的改变下增强或者减弱时微梁的涨落也分别相应的增大和减少。因此，可以通过将细胞吸附在微梁表面，利用这一现象实现对细胞的活性进行实时在线监测。本项目拟利用这一新的现象来实现对癌细胞活性的实时监测，并检测其在不同药物作用下活性的变化情况，从而实现微悬传感技术在细胞层次对抗癌药物的筛选。调控细胞新陈代谢的强弱，研究代谢的强弱和细胞活性与微梁涨落大小的关系。对细胞骨架蛋白进行标记，监测它们在相应的抑制药物作用下的变化情况（分布、动态性能和量的变化）以及微梁涨落的改变，从而研究微梁的涨落和细胞活性间内在联系的内在机理。

中文关键词： 微悬臂梁；细胞力学；表面应力；细胞活性；药物筛选

英文摘要： Looking for anticancer drug with high selectivity and small side effect is always one of the focus in the study of antitumor drugs. In the early of new era, a microcantilever sensor which is based on mechanical effect was developed. The principle of conventional microcantilever is based on the change of surface stress or the shift of resonance frequency. In our conventional research, we found that, in addition to this change and shift, the presence of living cell on microcantilever caused an obvious fluctuation of microcantilever, and when the activity was increased (or decreased), the fluctuation was also increased (or decreased). So, we can detect the cell activity in real-time and on-line using this new phenomena. Based on the new principle, this project prepares to detect the activity of cancer cells and the changes of the activity after treatment of different drugs to achieve antitumor drug screening. Through controlling the cell metabolism, we study the relation between the strength of the cell metabolism and the fluctuation. And for different cytoskeletal proteins of the cell, we add corresponding drug to suppress them, and then monitor the change (distribution, dynamic property and content) of proteins and the variation of the fluctuation to study the intrinsic mechanism of internal relation between the microcantilever fluctuation and the activity of cells.

英文关键词： microcantilever;cytomechanics;surface stress;cytoactive;drug screening