项目名称： 生物友好型磁光多功能纳米探针的制备、调控及生物检测

项目编号： No.21503004

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 张现峰

作者单位： 蚌埠学院

项目金额： 21万元

中文摘要： 磁光多功能纳米探针具有独特的磁性和光电等性质，在生物检测、生物成像和疾病诊断等领域有重要的应用前景。本项目基于磁性纳米粒子的磁性质和贵金属纳米粒子的局域表面等离子共振效应，设计磁性、表面增强荧光和表面增强拉曼散射三种分离检测技术于一体的磁光多功能纳米探针，将单个纳米材料的功能进行有效组合和放大，实现纳米探针的荧光信号和拉曼散射信号同时增强。采用化学性质稳定的低毒无污染荧光碳纳米材料作为荧光信号探针，有利于解决潜在的环境和健康问题。借助磁性和光学多模式检测和成像研究磁光多功能纳米探针对蛋白和肿瘤细胞的识别作用。通过本项研究，实现磁光多功能纳米探针结构、形貌和性能的有效控制，提供几种具有特定功能和应用前景的磁光多功能纳米探针，构建1-3种蛋白或肿瘤细胞识别检测方法，为肿瘤的早期检测和诊治提供新思路和参考依据。

中文关键词： 表面增强荧光；表面增强拉曼散射光谱；磁光多功能纳米探针；生物检测

英文摘要： Magneto-optical multifunctional nanoprobes, with unique properties of magnetic, optical and electrical, have important application prospect in the fields of biodetection, bio-imaging and disease diagnosis. This project, based on the magnetic property of magnetic nanoparticles and the localized surface plasmon resonance effect of noble metallic nanoparticles, will be implemented to design magneto-optical multifunctional nanoprobes which combine three kinds of separation and detection techniques of magnetic property, surface-enhanced fluorescence and surface-enhanced Raman scattering. The functionality of single nanomaterial will be effectively assembled and amplified, and the fluorescence signal and Raman scattering signal of nanoprobes can be enhanced simultaneously. Taking fluorescent carbon nanomaterials with a stable chemical properties and low toxicity as a fluorescent signal probe will help to resolve potential environmental and health problems. The recognition of magneto-optical multifunctional nanoprobes towards proteins and tumor cells will be studied by means of magnetic and optical multi-mode detection and imaging. The results of this project will not only realize the stable control of the structure, shape, size and functionality of the magneto-optical multifunctional nanoprobes, but also provide several multifunctional nanoprobes with specific functionality and application prospect, and will establish 1-3 proteins or tumor cell identification detection methods, as well as provide new ideas and reference for the early detection, diagnosis and treatment of tumors.

英文关键词： surface-enhanced fluorescence;surface-enhanced Raman scattering;；magneto-optical multifunctional nanoprobe;biodetection