Uncertain data without frequency stability often arises in experimental design. Classical fixed-effects models can only analyze precise experimental data. Based on an uncertain measure, this paper establishes uncertain fixed-effect models for Latin-square designs. First, we propose three methods with uncertainty to estimate the treatment and blocked effects and construct their confidence intervals. Then, uncertain homogeneity and common tests are conducted to assess the significance of treatment effects. In the numerical simulations, the three estimation methods are compared based on bias, mean squared error, mean absolute error, overall standard deviation, coverage probability, and average interval length. Several examples are given to illustrate the process of estimation and hypothesis. Finally, the uncertain fixed-effects model is applied to real education data, demonstrating its practical value.

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