Accurate phytoplankton segmentation is essential for ecological monitoring, yet it remains largely underexplored due to the subtle visual characteristics of phytoplankton and the scarcity of pixel-level annotations. To address these challenges, we propose PhySeg, a SAM-based segmentation framework equipped with a lightweight refinement module that progressively restores fine-grained details through multi-scale feature fusion. In addition, we construct a phytoplankton segmentation dataset by manually annotating instance-level masks for an existing dataset, providing a valuable resource for future research. Experimental results demonstrate that PhySeg improves segmentation accuracy, validating the effectiveness of the proposed module. Code is available at https://github.com/lijialin404/PhySeg.

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