项目名称： 褐飞虱味觉受体调控其繁殖力的分子机制研究

项目编号： No.U1401212

项目类型： 联合基金项目

立项/批准年度： 2015

项目学科： 管理科学

项目作者： 张文庆

作者单位： 中山大学

项目金额： 240万元

中文摘要： 高繁殖力是害虫经常暴发成灾的主要原因之一。昆虫繁殖力受内源性和外源性因素的共同影响。抗虫作物在一个世代内即能显著降低害虫的繁殖力，我们推测害虫味觉受体在其中发挥了重要作用。但是，参与调控害虫繁殖力的味觉受体及其调控机制均未见报道。本项目以水稻重要害虫褐飞虱为研究对象，在克隆其味觉受体基因家族的基础上，利用RNAi技术鉴定繁殖力相关味觉受体；研究这些受体对抗虫水稻中差异非挥发性次生化合物和糖类物质的响应，并在昆虫细胞中验证受体--配体关系；选择糖受体和响应非挥发性次生化合物的味觉受体各1个，深入研究受体通过转录因子作用于卵黄原蛋白(Vg)网络进而调控褐飞虱繁殖力的分子机制。本项目的创新之处在于：率先研究农业害虫味觉受体对抗虫作物活性物质的响应以及调控其繁殖力的分子机制。对于解释昆虫繁殖力的分子基础，加深对抗虫作物--害虫互作关系的理解均具有重要意义。

中文关键词： 褐飞虱；味觉受体；繁殖力；分子机制；水稻害虫

英文摘要： High fecundity is one of the major causes of insect outbreaks. Insect fecundity is affected by both endogenous and exogenous factors. Resistant crop varieties decrease fecundity of insect pests in a short period such as one generation in which insect gustatory receptors (GRs) are supposed to be involved. However, it is not reported which GRs are involved in the regulation of insect fecundity and how they regulate insect fecundity. Here, we will clone GR genes in the brown planthopper Nilaparvata lugens (BPH), a major insect pest of rice, and identify fecundity related GRs through RNAi. We will also study responses of the fecundity related GRs to non-volatile chemicals and saccharides differentially expressed in resistant rice varieties, and validate receptor-ligand relationships in insect cells. After selected one sugar receptor and one receptor responsed to non-volatile chemicals, we will study the molecular mechanisms of BPH fecundity regulated by the two receptors through pathways such as receptor-transcription factors-target genes in the Vg (vitellogenin) network-BPH fecundity. The novelty of this proposal is the pioneer study on GRs of agricultural insect pests, including their responses to active substances in resistant crop varieties and roles in the regulation of insect fecundity. The expected results will contribute to better understanding of molecular basis of insect fecundity and crop-insect interactions.

英文关键词： Nilaparvata lugens;gustatory receptor;fecundity;molecular mechanism;rice pest