The transition of cellular networks to (i) software-based systems on commodity hardware and (ii) platforms for services beyond connectivity introduces critical system-level challenges. As sensing emerges as a key feature toward 6G standardization, supporting Integrated Sensing and Communication (ISAC) with limited bandwidth and piggybacking on communication signals, while maintaining high reliability and performance, remains a fundamental challenge. In this paper, we provide two key contributions. First, we present a programmable, open-source framework for processing PHY/MAC signals through real-time, GPU-accelerated Artificial Intelligence (AI) applications on the edge Radio Access Network (RAN) infrastructure. Building on the Open RAN dApp architecture, the framework interfaces with a GPU-accelerated gNB based on NVIDIA Aerial Testbed (ATB), feeding PHY/MAC data to custom AI logic with a framework overhead of 150 us, multiple inference engines, and support for several AI backends. We evaluate the framework on multiple GPU platforms with and without hardware-level GPU isolation. Second, we demonstrate the framework capabilities through cuSense, an indoor localization dApp that consumes uplink DMRS channel estimates, removes static multipath components, and runs a neural network to infer the position of a moving person. Evaluated on a 3GPP-compliant 5G NR deployment, cuSense achieves a mean localization error of 77 cm, with 75% of predictions falling within 1 meter, without dedicated sensing hardware or modifications to the RAN stack or signals. The framework is released as open source, providing a reference design for future AI-native RANs and ISAC applications.

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