Virtual-cell and perturbation models are increasingly used to predict cellular responses for biomedical discovery, but chemical and genetic perturbations are not automatically interchangeable. Existing evaluations often study chemical response prediction or genetic perturbation prediction separately, leaving target-matched chemical-to-genetic translation under-tested. We introduce Chem2Gen-Bench, a benchmark comprising 260,084 chemical and 1,099,045 genetic perturbation profiles organized into cell-target contexts, and evaluate pairwise alignment, retrieval, protocol covariate associations, feature spaces, and foundation-model embeddings. Across matched contexts, translation fidelity is measurable but heterogeneous; background adjustment increases the association between pairwise similarity and retrieval success, while paired tests show lower mean retrieval success after adjustment under the evaluated settings. In a target-matched K562 audit, the evaluated foundation-model embeddings did not consistently improve over gene-delta baselines. Chem2Gen-Bench provides an auditable framework for testing when chemical and genetic perturbations align around shared targets and when representation gains are supported by matched perturbation evidence.

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