Large Language Models (LLMs) are increasingly used as recommendation generators in conversational recommender systems (RS) via retrieval-augmented generation (RAG). This flexible design introduces new sources of structural bias. This paper studies positional bias in top-1 and single-turn LLM-based RS: systematic dependence of the recommended item on the order in which candidates are presented in the prompt. We propose two complementary detection methodologies: (i) POB-ICM, based on contingency matrix analysis and Chi-square independence testing, and (ii) POB-UNBP, a sample-efficient approach derived from a uniformity null model under permutation-based evaluation, enabling stochastic decoding with formal statistical guarantees. Using a controlled RAG testbed for product recommendation, built on a real product catalog, a fixed prompt template and 2000 template-generated user queries with 20 permutations each (40,000 instances), both methodologies reject the null hypothesis of positional independence and uniformity in all evaluated state-of-the-art models. We introduce three complementary metrics -- permutation sensitivity, positional bias, and strong positional bias -- to quantify this effect, the latter targeting cases where the position displaces the recommendation towards clearly less relevant items rather than only choosing among comparably relevant ones. A robustness analysis across candidate-set size and decoding temperature shows that the bias is structural and model-dependent, not an artifact of deterministic decoding or candidate-set size. These results establish positional bias as a measurable, reproducible property of prompt-mediated recommendation pipelines in the evaluated setting and motivate routine auditing and governance controls (e.g., order randomization, standardized prompting) for deployed LLM-based RS. Source code is publicly released.

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